Effect of different extraction solvents on the yield and enzyme inhibition (a-amylase, a-glucosidase, and lipase) activity of some vegetables

Abstract. Maser WH, Maiyah N, Nagarajan M, Kingwascharapong P, Senphan T, Ali AMM, Bavisetty SCB. 2023. Effect of different extraction solvents on the yield and enzyme inhibition (a-amylase, a-glucosidase, lipase) activity some vegetables. Biodiversitas 24: 3320-3331. The present study investigated in vitro a-amylase, lipase, antioxidant activities (DPPH, metal chelating, FRAP) Allium cepa L., Apium graveolens Coriandrum sativum Petroselinum crispum (Mill.) Fuss using solvent 80% ethanol, absolute acetone, methanol, n-hexane, chloroform, hot water. total phenolic content (TPC) FTIR GC-MS characterization spectra were compared with activities. TPC ethanol extract A. L. was highest among extracts, 23.78 mg GAE/g extract. exhibited most potent DPPH radical scavenging (A. graveolens, 125.57 AEAC/g extract), chelating (C. sativum, 92.85 EECC/g FRAP 46.98 extract). showed anti-a-amylase (P. crispum, 30.61 mmol ACE/g extract) anti-a-glucosidase cepa, 595.28 In comparison, C. anti-lipase (42.10% inhibition). According to spectra, four green leafy vegetables is predicted have several active compounds. identified compounds responsible for activity. Studies recovery potential as oral agents treat diabetes mellitus certain are very useful producing functional foods prevent mellitus.